Effects of Insufficient Sleep on Circadian Rhythmicity and Expression Amplitude of the Human Blood Transcriptome

Twenty-six participants were exposed to 1 week of insufficient sleep (mean 5.7 hours/24 hours) and 1 week of sufficient sleep (mean 8.5 hours/ 24 hours). 10 whole blood RNA samples were obtained from each participant. Transcriptome analyses revealed 711 genes up and down-regulated in response to the sleep restriction condition, including genes implicated in circadian rhythm, sleep homeostasis, oxidative stress, and metabolism. Identified pathways may help elucidate mechanisms that underlie associations between insufficient sleep and disease risk.